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Larger Image Fig. 1. In situ hybridization histochemistry showing the distribution of TRH precursor mRNA in the brain and pituitary of Xenopus laevis. AâF: Frontal brain sections from white-adapted frogs were hybridized with an antisense TRH precursor riboprobe and exposed onto Hyperfilm max for 8 days. The anatomical structures, identified by microscopic analysis, are designated on the right hemisections. G: A control section incubated with a sense riboprobe (right hemisection) is compared with a consecutive section hybridized with the antisense probe (left hemisection). The rostrocaudal levels of the sections are indicated on the schematic representation of the brain. Abbreviations as in Table 1. Scale bars 1 mm. Image published in: Bidaud I et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher.
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