Fig. 3 Expression of Na,KATPase a1 and g subunit genes during Xenopus embryogenesis. Whole-mount in situ hybridizations were performed with antisense probes for a1 and g subunits. Transverse sections of stage 28 (K, L) were cut at 30â40 mm. Lateral views (A-H) are shown with anterior to the left. Frontal views (I, J) and sections (K, L) are oriented with dorsal to the top. A-D a1 subunit expression was found throughout the epidermis of tailbud embryos (A, stage 23; B, stage 26; and C, stage 28). Expression in the pronephric anlage can be anticipated from stage 26 on (white arrowheads). At stage 37, a1 transcripts were prominently detected in the olfactory bulbs, otic vesicles, the gills (arrowheads), the pronephric kidneys, and facial (VII) and glossopharyngeal (IX) nerves. E-H Stage 22 embryo (E) showed a punctate staining pattern for the g subunit. At stage 25 (F), g expression was overall low, but became at stage 28 (G) apparent in the brain, eyes, otic vesicles, and pronephric primordia. In the stage 38 embryo (H), strong expression of g transcripts was confined to the pronephric kidneys. I, J Frontal views of stage 37/38 embryos stained for a1 and g expression. Strong staining for a1 transcripts (J) was seen in the olfactory bulbs. K, L Transverse sections of stage 28 embryos revealed a1 (K) in the epidermis and g (L) expression in the pronephric and hepatic primordia. For abbreviations, see legend to Fig. 2.
Image published in: Eid SR and Brändli AW (2001)
Copyright © 2001. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| atp1a1.L | atp1a1-a, atp1a1-b, K-ATPase, Na+/K+ ATPase, Na+K+ ATPase, Na+K+ATPase | X. laevis | Sometime during NF stage 24 to NF stage 37 and 38 | pronephric kidney epidermis |
| fxyd2.S | Na,K-ATPase gamma | X. laevis | Sometime during NF stage 28 to NF stage 39 | pronephric mesenchyme pronephric kidney pronephric tubule proximal tubule distal tubule brain eye heart primordium |
Image source: Published
Permanent Image Page
Printer Friendly View
XB-IMG-152460