Fig. 3. WISH analysis of PGC transcripts. Expression of selected PGC-enriched mRNAs at post-MBT st. 11.5 (gastrula), 16 (neurula) and 33/34 (tailbud). pgat expression marks germ plasm in PGCs. Transcripts detected in PGCs at neurula are shown at higher magnification in insets. WISH analysis was performed on â¥21 total embryos from at least three adult female frogs. Insets show magnifications of the boxed areas. A, anterior; b, brain; ba, brachial arches; cg, cranial ganglia; D, dorsal; gl, cement gland; isr, intersegmental region; L, left; n, notochord; np, nasal placodes; nt, neural tube; ov, otic vesicle; P, posterior; pn, pronephros; R, right; s, somites; V, ventral; vbi, ventral blood islands; Vg, vegetal view. Scale bars: 200 μm.
Image published in: Butler AM et al. (2018)
Copyright © 2018. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| cdc20.L | fizzy1, X-FZY | X. laevis | Throughout NF stage 11.5 | marginal zone |
| fam168b.L | X. laevis | Throughout NF stage 11.5 | marginal zone germ plasm | |
| fer.L | X. laevis | Throughout NF stage 11.5 | marginal zone germ plasm | |
| lmnb3.L | lamin b3, lamin-b3, lamin LIII, LB3 | X. laevis | Throughout NF stage 11.5 | germ plasm |
| nasp.L | X. laevis | Throughout NF stage 11.5 | germ plasm | |
| pgam1.L | X. laevis | Throughout NF stage 11.5 | germ plasm | |
| rtn3.L | asyip, hap, nspl2, nsplii, rtn3-a, rtn3-a1, rtn3-b, xrtn3 | X. laevis | Throughout NF stage 11.5 | germ plasm marginal zone |
| pphln1.L | ga50, periphilin, periphilin-1, periphilin1 | X. laevis | Throughout NF stage 11.5 | marginal zone |
| tspan1.L | tetraspanin-1 | X. laevis | Throughout NF stage 11.5 | ventral marginal zone germ plasm |
| ppp1r2.L | X. laevis | Throughout NF stage 11.5 | germ plasm | |
| prpsap2.S | LOC108701659, LOC108703217 | X. laevis | Throughout NF stage 11.5 | germ plasm |
| pgat.L | Xpat | X. laevis | Sometime during NF stage 11.5 to NF stage 33 and 34 | primordial germ cell |
| cdc20.L | fizzy1, X-FZY | X. laevis | Throughout NF stage 16 | germ plasm |
| fam168b.L | X. laevis | Throughout NF stage 16 | ectoderm germ plasm | |
| fer.L | X. laevis | Throughout NF stage 16 | germ plasm | |
| lmnb3.L | lamin b3, lamin-b3, lamin LIII, LB3 | X. laevis | Throughout NF stage 16 | germ plasm ectoderm |
| nasp.L | X. laevis | Throughout NF stage 16 | germ plasm ectoderm | |
| pgam1.L | X. laevis | Throughout NF stage 16 | germ plasm | |
| rtn3.L | asyip, hap, nspl2, nsplii, rtn3-a, rtn3-a1, rtn3-b, xrtn3 | X. laevis | Throughout NF stage 16 | germ plasm ectoderm |
| pphln1.L | ga50, periphilin, periphilin-1, periphilin1 | X. laevis | Throughout NF stage 16 | germ plasm |
| tspan1.L | tetraspanin-1 | X. laevis | Throughout NF stage 16 | germ plasm |
| ppp1r2.L | X. laevis | Throughout NF stage 16 | germ plasm | |
| prpsap2.S | LOC108701659, LOC108703217 | X. laevis | Throughout NF stage 16 | germ plasm |
| cdc20.L | fizzy1, X-FZY | X. laevis | Throughout NF stage 33 and 34 | pharyngeal region head trunk eye |
| fam168b.L | X. laevis | Throughout NF stage 33 and 34 | pharyngeal region eye brain trunk | |
| fer.L | X. laevis | Throughout NF stage 33 and 34 | pharyngeal region head trunk | |
| lmnb3.L | lamin b3, lamin-b3, lamin LIII, LB3 | X. laevis | Throughout NF stage 33 and 34 | somite brain ventral trunk eye pharyngeal region |
| nasp.L | X. laevis | Throughout NF stage 33 and 34 | head pharyngeal region trunk | |
| pgam1.L | X. laevis | Throughout NF stage 33 and 34 | somite notochord otic vesicle cranial ganglion brain pharyngeal region eye | |
| rtn3.L | asyip, hap, nspl2, nsplii, rtn3-a, rtn3-a1, rtn3-b, xrtn3 | X. laevis | Throughout NF stage 33 and 34 | ventral blood island intersomitic region cranial ganglion eye branchial arch brain |
| pphln1.L | ga50, periphilin, periphilin-1, periphilin1 | X. laevis | Throughout NF stage 33 and 34 | pronephric kidney eye brain |
| tspan1.L | tetraspanin-1 | X. laevis | Throughout NF stage 33 and 34 | cranial ganglion cement gland pronephric kidney |
| ppp1r2.L | X. laevis | Throughout NF stage 33 and 34 | cranial ganglion | |
| prpsap2.S | LOC108701659, LOC108703217 | X. laevis | Throughout NF stage 33 and 34 | eye pharyngeal region pronephric kidney head spinal cord |
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