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Fig. 3. WISH analysis of PGC transcripts. Expression of selected PGC-enriched mRNAs at post-MBT st. 11.5 (gastrula), 16 (neurula) and 33/34 (tailbud). pgat
expression marks germ plasm in PGCs. Transcripts detected in PGCs at neurula are shown at higher magnification in insets. WISH analysis was performed on
â¥21 total embryos from at least three adult female frogs. Insets show magnifications of the boxed areas. A, anterior; b, brain; ba, brachial arches; cg, cranial
ganglia; D, dorsal; gl, cement gland; isr, intersegmental region; L, left; n, notochord; np, nasal placodes; nt, neural tube; ov, otic vesicle; P, posterior; pn,
pronephros; R, right; s, somites; V, ventral; vbi, ventral blood islands; Vg, vegetal view. Scale bars: 200 μm.