Fig. 1. In vitro assays of the absorption spectra of the wild-type and mutant bovine RH1 pigments. (A) The opsin cDNAs in an expression vector, pMT5, are expressed in COS1 cells by transient transfection. (B) The visual pigments were then regenerated by incubating the opsins with 11-cis-retinal in the dark. (C) The resulting visual pigments are then purified by immunoaffinity chromatography by using monoclonal antibody 1D4 Sepharose 4B. The absorption spectra of the visual pigment are recorded using a spectrophotometer. (D) The amino acid change A292S has been obtained by site-directed mutagenesis. The absorption spectrum in inset shows the λmax determined by dark-light spectrum.
Image published in: Yokoyama S (2002)
Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V.
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