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Int J Mol Sci 2024 Jan 10;252:. doi: 10.3390/ijms25020870.
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Mechanical Tensions Regulate Gene Expression in the Xenopus laevis Axial Tissues.

Eroshkin FM , Fefelova EA , Bredov DV , Orlov EE , Kolyupanova NM , Mazur AM , Sokolov AS , Zhigalova NA , Prokhortchouk EB , Nesterenko AM , Zaraisky AG .

During gastrulation and neurulation, the chordamesoderm and overlying neuroectoderm of vertebrate embryos converge under the control of a specific genetic programme to the dorsal midline, simultaneously extending along it. However, whether mechanical tensions resulting from these morphogenetic movements play a role in long-range feedback signaling that in turn regulates gene expression in the chordamesoderm and neuroectoderm is unclear. In the present work, by using a model of artificially stretched explants of Xenopus midgastrula embryos and full-transcriptome sequencing, we identified genes with altered expression in response to external mechanical stretching. Importantly, mechanically activated genes appeared to be expressed during normal development in the trunk, i.e., in the stretched region only. By contrast, genes inhibited by mechanical stretching were normally expressed in the anterior neuroectoderm, where mechanical stress is low. These results indicate that mechanical tensions may play the role of a long-range signaling factor that regulates patterning of the embryo, serving as a link coupling morphogenesis and cell differentiation.

PubMed ID: 38255964
PMC ID: PMC10815341
Article link: Int J Mol Sci
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: adamts7 cd82 cdx4 cebpa cer1 foxc1 foxc2 frzb2 fst gbx2 gsc irx3 lef1 mmp21 myf5 nalcn otx2 prss8l.4 sfrp1 shh six3 socs3 tbx6 tbxt xmc
GO keywords: neural tube closure [+]

Phenotypes: Xla Wt + neurectoderm explant + stretch (Fig. 3. B)

Article Images: [+] show captions
References [+] :
Agricola, Identification of genes expressed in the migrating primitive myeloid lineage of Xenopus laevis. 2016, Pubmed, Xenbase