Xenbase Image ID: 125913

Figure 2. Downregulation of miR-124 increases the expression of NeuroD1 in the optic vesicle. (A) Expression of NeuroD1 increased in stages 20 and 22/23 embryos but not in stages 33/34 embryos injected (Inj.) with Anti-124, as indicated by in situ hybridization, while expression of Lhx2 was not affected at any of these stages. Yellow dashed lines indicate the midlines of Stage 20 embryos. Red dots circle the position of the optic vesicle/cup. Scale bar: 500 μm. (B) RT–PCR and real-time PCR quantification confirm that expression of NeuroD1, but not Lhx2, in stage 22/23 embryos is upregulated on loss of miR-124. (C) Correspondingly, the expression of erC increased and that of xtwi decreased. *P and #P < 0.05; **P and ##P < 0.01. Image published in: Liu K et al. (2011) © The Author(s) 2010. Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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