XB-IMG-125605
Xenbase Image ID: 125605
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Figure 3. S2 formation is divalent metal dependent and requires HMGN elements outside of the NBD. EMSA gel labels indicate HMGN identity (N1t, HMGN1t; N1, HMGN1; N2, HMGN2; a, 12-array alone; n, NCP alone), and numbers designate HMGN : nucleosome molar stoichiometry. (a) HMGN binding to 12-array at near physiological ionic strength with 1 mM Ca2+. (b) Binding of HMGN proteins and NBD peptides to NCP-601 at near physiological ionic strength with 1 mM Mg2+. (c) Binding of HMGN NBD peptides to 12-array at low ionic strength (top), near physiological ionic strength (middle), and near physiological ionic strength with 1 mM Mg2+ (bottom). Image published in: Ong MS et al. (2010) Image downloaded from an Open Access article in PubMed Central. Copyright © 2010 Michelle S. Ong et al. Larger Image Printer Friendly View |