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Figure 11. Apparent affinities for activation of channel Po by MgATP. Representative macropatch currents are shown for WT and Flag-WT (A), 1-633+634-1480 and Flag3-633+634-1480 (B), 1-432+433-1480 and Flag3-432+433-1480 (C), and Flag3-414+433-1480 (D) channels. After prephosphorylation by PKA, and removal of both PKA and ATP, once all channels had closed, 50 μM ATP was applied for 10–30 s, bracketed between exposures to 2 mM ATP. (E) The ratios of the mean steady currents, I50μM/I2mM, were 0.50 ± 0.02 (WT), 0.56 ± 0.03 (Flag-WT), 0.51 ± 0.02 (1-633+634-1480), 0.42 ± 0.01 (Flag3-633+634-1480), 0.49 ± 0.02 (1-432+433-1480), 0.44 ± 0.01 (Flag3-432+433-1480), and 0.45 ± 0.01 (Flag3-414+433-1480). None of the constructs differed significantly from WT at P < 0.05.

Image published in: Chan KW et al. (2000)

© 2000 The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license

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