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Figure 4. ‘One pot assay’ of conventional and H2AL2 nucleosomes. (A) Kinetics of the HaeIII digestion of conventional H2A (Nuc H2A) and variant H2AL2 (Nuc H2AL2) nucleosomes. Identical amounts of both types of nucleosomes were digested with 5 U/µl of Hae III at 30°C for the times indicated. After arresting the digestion, DNA was isolated and run on an 8% denaturing PAGE. Lane 1, naked DNA digested with 5 U/µl for 5 min. (B) Quantification of the data presented in (A). Note that the quantification for the accessibility at d7 is not presented, since the corresponding band is not resolved from the undigested DNA under our conditions (see panel A). Image published in: Syed SH et al. (2009) © 2009 The Author(s). Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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