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Figure 7. Relative expression of T cell–related immune genes in the liver of M. marinum–inoculated adults and tadpoles. Four-month-old young adults (white bar) and 3-wk-old tadpoles (gray bar) were i.p. inoculated with 1 × 106 or 3 × 105 CFU of M. marinum, respectively (n = 5–6 per time point). Relative gene expression in liver for (A) CD3ε, (B) CD8β, (C) CD4, and (D) iVα45-Jα1.14 was normalized to the housekeeping gene gapdh. (E) Ct values for gapdh of each time point between adults and tadpoles. Asterisks indicate a significant difference by the Kruskal–Wallis test (p < 0.05). C, uninfected control; u.d, undetected value.

Image published in: Rhoo KH et al. (2019)

Copyright © 2019. Image reproduced with permission of the Publisher.

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