XB-IMG-125592
Xenbase Image ID: 125592
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Figure 2. The competence of Nnat overexpressing and knockdown ESCs to give rise to the three primary germ cells. (A): Quantitative RT-PCR analysis for markers of the three primary germ cells using RNAs derived from control, Nnatα-OE, and Nnat-KD ESCs using an embryoid body (EB) formation assay. The total RNA from each ESC line was collected from 6-day differentiated EBs and the cDNAs were used to analyze the various cell markers: Fgf5 (primitive ectodermal cells), Hnf4 (endodermal cells), T and Mesp (mesodermal cells), Otx2 (ectodermal cells), and Six3 and Sox1 (neuroectodermal cells). Data shown are the mean ± SD (n = 3). *, p < .05 and **, p < .01, significantly different from the control group; #, p < .05 and ##, p < .01, significantly different from the Nnatα-OE group, two-tailed Student's t test. (B): ICC analysis of the mesodermal and epidermal cells derived from control, Nnatα-OE, and Nnat-KD ESCs identified by staining with T and Krt18, respectively. Scale bar = 50 μm. All nuclei were stained with DAPI (blue). Abbreviations: DAPI: 4â²,6â²-diamidino-2-phenylindole; ICC, immunocytochemical; Nnatα-OE, neuronatin α-overexpression; Nnat-KD, neuronatin-knockdown; RT-PCR: real time-polymerase chain reaction. Image published in: Lin HH et al. (2010) Copyright © 2010 AlphaMed Press. Creative Commons Attribution license Larger Image Printer Friendly View |