XB-IMG-128646
Xenbase Image ID: 128646
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Figure 2. a) Concentration dependence of compound 1 for tyrosinase oxidation DNA alkylating were incubated with 5′-end TAMRA labeled Pu27 DNA in 10 mM Tris/HCl buffer at pH 7.0 for 1 h at 37°C. The amounts of labeled DNA and tyrosinase were fixed as 10 pmol and 40 Units, respectively. Lane 1–3 are control lanes, lane 4–11 are increasing concentrations of compound 1 (10 μM, 20 μM, 50 μM, 0.1 mM, 0.2 mM, 0.5 mM, 1 mM, 2 mM) incubation with labeled Pu27 DNA and tyrosinase (40 Units). The alkylated oligo was separated from the nonreacted DNA by 20% denaturing polyacrylamide gel. b) Compound 1 (1 mM) was incubated with labeled Pu27 DNA (10 pmol) and tyrosinase (40 Units) in the presence of increasing molar ratios (0.5, 1, 2, 5, 10) of unlabeled Pu27 DNA(G4) or unlabeled ds-Pu27 DNA(double-stranded, ds) for 1 h at 37°C. The alkylated oligo was separated from the nonreacted DNA by 20% denaturing polyacrylamide gel. “C” refers to labeled Pu27 DNA (10 pmol) treated at 37°C for 1 h, and “R” refers to the Pu27-compound 1 reaction product in the absence of any competitor DNA. Image published in: Yuan L et al. (2013) Copyright © 2013, Macmillan Publishers Limited. Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View |