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XB-IMG-178521

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Figure S10: Chip-qPCR confirms increased H2B ubiquitination in ciliated cells (A) The H2Bub1 binding profiles, depicted using fold enrichment against random distribution values ranging from 0 to 10, across the eight most significant cilia genes based on enrichment of H2Bub1 occupancy in ciliated tissue (oviduct, shown in red) compared to non-ciliated tissue (liver, shown in blue) according to Chip-seq. The arrows indicate the transcription start site. (B) ChIP-qPCR of mouse oviducts and liver with anti-H2Bub1 antibody. qPCR primers: NC (non-specific primers), GAPDHp (negative control), GAPDHc (positive control), RFX3A and RFX3B are RFX3 specific primers; blue boxes are 5'UTR, red boxes are the coding region. Chip qPCR was performed in ciliated (oviducts-red) and nonciliated (liver-blue) tissues from Rnf20+/+ and Rnf20+/- mice. Results are from 4 independent experiments, unpaired T-test, ***p<0.0005.

Image published in: Robson A et al. (2019)

Copyright © 2019. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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