XB-IMG-128986
Xenbase Image ID: 128986
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Figure 1. Pol κ is required for Chk1 phosphorylation after replication stress in human cells. (A, B) Western blot analysis of cell extracts from Human U2OS and MRC5 untransfected (unt) or transfected with control luciferase siRNA (si-luc), Pol κ siRNA (si-κ1, si-κ2, D-si-κ), untreated or treated with HU (2 mM, 3 h). Cell extracts were fractionated and soluble fractions were analysed with the indicated antibodies. Quantification was performed by ImageJ software; means±s.d. were presented. (C) Western blot analysis of whole cell extracts prepared from 293T cells treated with HU (2 mM, 3 h) transfected by si-RNA alone (si-luc or si-κ3'UTR) or co-transfected by si-κ3′UTR and a vector expressing FLAG-tagged wild-type Pol κ (κ-WT). (D, E) Pol η depletion with Pol η siRNA (si-η) or transfection with vector expressing the FLAG-tagged catalytic inactive mutant of Pol κ (Pol κ-Dead) was performed in MRC5 cells untreated or treated with HU (2 mM, 3 h).Source data for this figure is available on the online supplementary information page.Source Data for Figure 1. Image published in: Bétous R et al. (2013) Copyright © 2013, European Molecular Biology Organization. Creative Commons Attribution license Larger Image Printer Friendly View |