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Fig. 4. The Cat-S inhibitor LHVS has no effect on ENaC activation by the serine proteases trypsin and chymotrypsin. a Oocytes expressing human ENaC were incubated for 30 min in protease-free solution (control), in solutions with different concentrations of chymotrypsin (0.02, 0.2, and 2 μg/ml), in LHVS (2 μM), or in solutions containing a combination of different concentrations of chymotrypsin (0.02, 0.2, and 2 μg/ml) and LHVS (2 μM). Amiloride-sensitive whole-cell currents (ΔIami) were determined before (−) and after (+) incubation. Columns represent relative stimulatory effect on ΔIami calculated as the ratio of ΔIami measured after 30 min of incubation (ΔIami 30 min) to the initial ΔIami (ΔIami initial) measured before incubation. Numbers inside the columns indicate the number of individual oocytes measured. b Similar experiment as shown in a using the serine protease trypsin (2 μg/ml) instead of chymotrypsin Image published in: Haerteis S et al. (2012) Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2012 Larger Image Printer Friendly View

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