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Figure 7. . Dependence on [PKA] of open probability, Po, of WT (•) and S768A (○) CFTR channels. Steady-state mean Po measured in (n) excised patches, containing few channels, during exposure to 6–550 nM PKA plus near-saturating [MgATP] (1–2 mM), is plotted against [PKA]. Lines show nonlinear least-squares fits to the Hill equation, yielding Po,max = 0.34 ± 0.06, K0.5 = 149 ± 46 nM, nH = 1.5 ± 0.5 for WT, and Po,max = 0.51 ± 0.05, K0.5 = 71 ± 12 nM, nH = 1.8 ± 0.5 for S768A.

Image published in: Csanády L et al. (2005)

Copyright © 2005, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license

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