XB-IMG-124890
Xenbase Image ID: 124890
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Figure 7. Paxilline sensitivity is restored in constructs with a Slo3 S6 by a glycine at position S300. (A) Paxilline blocks MC10-S300G. 50 nM paxilline markedly inhibits MC10-S300G currents activated at +150 mV with 0 Ca2+. (B) G-V curves are plotted for MC10-S300G with and without 50 nM paxilline (n = 4 patches). Red lines are a fit of Eq. 2 with Kb = 13.1 ± 0.6 nM and no voltage dependence. (C) Paxilline more weakly blocks MC13-S300G. Traces show MC13-S300G currents activated at pH 8.5 at +200 mV. Inhibition occurs with high concentrations of paxilline. (D) G-V curves are plotted for MC13-S300G with and without paxilline (n = 3 patches). Red lines are a fit of Eq. 2 with Kb = 170.2 ± 7.2 nM and no voltage dependence. Image published in: Zhou Y et al. (2010) © 2010 Zhou et al. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |