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XB-IMG-117431

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Fig. 4. Immunoblotting reveals no constitutive phosphorylation of HEK293 cell-expressed P2X2 and P2X3 receptors. The indicated proteins were isolated by immunoprecipitation using the peptide-specific P2X3 subunit polyclonal antibody or an EGFP-specific polyclonal antibody as appropriate and resolved by reducing SDS-PAGE. a Shown is an immunoblot probed by phosphothreonine-specific monoclonal antibody and a peroxidase-conjugated secondary antibody. Note that phosphorylation of the P2X3 receptor or the EGFP-tagged P2X2 receptor is not detectable, whereas a positive control—the EGFP-tagged splicing factor SF3B1 (migration mass 97 kDa [23])—was detected by the antibody. b The same samples as in panel a were immunoblotted using peptide-specific P2X2 and P2X3 subunit polyclonal antibodies to verify the expression of the transfected genes

Image published in: Franklin C et al. (2007)

Image downloaded from an Open Access article in PubMed Central. © Springer Science + Business Media B.V. 2007

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