XB-IMG-80417
Xenbase Image ID: 80417
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Fig. 5.
Morpholino knock-down of Shroom2 results in severe RPE hypopigmentation. (A) RT-PCR confirmation of morpholino efficacy. Normally spliced Shroom2 mRNA was absent from 80 ng Shroom2-SPL-MO injected embryos. (B) Shroom2-SPL-MO or Shroom2-5MM-MO (80 ng) was injected into two animal blastomeres at the four-cell stage and embryos were grown to stage 42. (C,D) Shroom2-SPL-MO injected embryos show severe reduced RPE pigmentation (E,e′) when compared with stage-matched uninjected or Shroom-5MM-MO embryos (C-d′). (F) Measurement of pixel density in the eye indicates no significant difference in pigmentation between uninjected and Shroom2-5MM-MO-injected embryos at stage 39, but a drastic difference between pixel density between controls and Shroom2-SPL-MO eyes. Image published in: Fairbank PD et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |