XB-IMG-122895
Xenbase Image ID: 122895
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Figure 3. SINBAD for the detection of protein-DNA interactions.(A) his-TRF2 was immobilized on Ni-NTA beads and incubated with hybridized 5â²-biotinylated DNA oligos containing three repeats of TTAGGG (red), a mutant TTACGG or ssTTAGG as indicated. Bound DNA was visualized with streptavidin-coated QDs655. (B) <10 his-TRF2 Ni-NTA beads bound to wild-type telomere sequence were incubated with increasing concentrations of competitor DNA oligos. Fluorescence intensity of QD655 was determined from confocal images and plotted. Image published in: Schulte R et al. (2008) Hetzer et al. Creative Commons Attribution license Larger Image Printer Friendly View |