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Figure 9. Anti-CBsv antibodies are specific for the Sphaerechinus granularis splice variant of cyclin B. 20-h-old embryos of S. granularis (a and b) or Paracentrotus lividus, another species of regular sea urchin, were methanol-fixed and processed for cyclin B detection with a polyclonal antibody raised against full-length Arbacia punctulata cyclin B (a and c) or for CBsv detection with the anti-CBsv antibody (b and d). Anti–cyclin B antibody clearly detected the overexpressed CBsv with a similar pattern in both species, even in the presence of 1 mg/ml CBsv peptide (a and c). In contrast, anti-CBsv antibody, which is directed against a specific intronic sequence in S. granularis, did not recognize any epitope in the related species P. lividus (b and d). Bar, 50 μm. Image published in: Lozano JC et al. (1998) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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