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FIGURE 5. Expression of TOM1. A, regulation of TOM1 mRNA levels by Fe availability in rice. The upper panel shows the Northern blot analysis, and the lower panel shows the quantitative RT-PCR analysis of TOM1. +, Fe-sufficient; −, Fe-deficient. In the Northern blot analysis, ethidium bromide-stained rRNA is shown as a loading control. In the quantitative real time PCR analysis, the values were normalized with the expression of OsActin1 and represent the mean ± S.D. (error bars) from three reactions. B, diurnal changes in TOM1 expression in Fe-deficient rice roots. L, light; D, dark. C, diurnal changes in HvTOM1 expression in Fe-deficient (−) and Fe-sufficient (+) barley roots. L, light; D, dark.

Image published in: Nozoye T et al. (2011)

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc. Creative Commons Attribution-NonCommercial license

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