Xenbase Image ID: 116979

Figure 2. The 3′ BC1 domain is repression competent. (A) Luciferase mRNA (Luc) was coinjected with 100 nM of small RNAs into stage VI oocytes and incubated for 55 min. Results from four experiments were quantified by luminescence (one-way ANOVA, P < 0.001; Scheffe's multiple comparison post hoc analysis [comparison with Luc control]: **, P < 0.01; *, P < 0.05). (B and C) 48S complex formation was analyzed in the presence of the 3′ (B) or the 5′ (C) BC1 domain (500 nM), using capped polyadenylated α-tubulin mRNA as a programming mRNA. (D and E) Analogously, 48S complex formation was probed in the presence of the 3′ (D) or the 5′ (E) BC1 domain, using uncapped polyadenylated CSFV.NS' mRNA as a programming mRNA. Image published in: Wang H et al. (2005) Copyright © 2005, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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