XB-IMG-116851
Xenbase Image ID: 116851
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Figure 5. Xenopus Pol ε holoenzyme interacts directly with xGINS, xCdc45p, and xCut5p in vitro. (A) Flag-tagged r-xPol ε holoenzyme was incubated with xGINS [29] for 1 h at 4°C and immunoprecipitated with anti-Flag antibody. The immunoprecipitates were analyzed by SDS-PAGE followed by Western blotting. IP; immunoprecipitates, SUP; supernatant. (B) Increasing concentrations of xCut5p were incubated with xGINS and Flag-tagged xCdc45 [30] or Flag-tagged BAP for 1 h at 4°C and immunoprecipitated as in (A). (C) r-xPol ε holoenzyme was pre-incubated with either Flag-tagged xGINS or Flag-tagged xCdc45p prior to addition of the indicated protein(s). Reactions were analyzed as in (A). (D) A model for the interaction between r-xPol ε and replication accessory proteins (xGINS, xCdc45, and xCut5). Image published in: Shikata K et al. (2006) Copyright © 2006 Shikata et al; licensee BioMed Central Ltd. Creative Commons Attribution license Larger Image Printer Friendly View |