Xenbase Image ID: 123317

Figure 1. Effects of intracellular nucleotides on the common gate Poc-V curve of wild-type CLC-1 and two single-point mutants. All experiments were performed with 3 mM nucleotides/nucleosides at a pHi of 6.2. Poc was determined using protocol A. (A) Comparison of the effects of various nucleotides/nucleosides on the Poc-V curve of CLC-1 channels expressed in Xenopus oocytes. Results on the left (n = 3) and right (n = 4) were obtained from different sets of membrane patches. (B) Effects of 3 mM ATP on the Poc-V curve of the H847A mutant (expressed in oocytes). Black squares, before ATP application; red squares, 3 mM ATP; small open triangles, after ATP washout (n = 4). For comparison, the Poc-V curves of the wild-type CLC-1 shown in the right panel of A are plotted as black (control) and red curves (3 mM ATP). (C) Effects of 3 mM ATP on the steady-state Poc-V curve of the L848A mutant (expressed in oocytes). The Poc-V curves of the wild-type CLC-1 in the absence (control) and in the presence of 3 mM ATP are the same as in B. A smaller ATP effect in the mutant compared with that of the wild-type CLC-1 was also observed in experiments using tsA201 cells. Image published in: Zhang XD et al. (2008) © 2008 Zhang et al. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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