Xenbase Image ID: 178448

Figure 3. Xpod is required for MucXS and serotonin release. Xpod function was analyzed by the XpodMO‐mediated gene knockdown. (a–c) MucXS and serotonin loaded granules were detected by immunofluorescence using PNA (red) and anti‐serotonin antibody (green), respectively. Cell borders were visualized by actin staining (phalloidin, blue). (a–c) Controls (co.; a) showed MucXS and serotonin positive granules, which were reduced in Xpod morphants (b). Co‐injecting XpodMO and rescue‐Xpod mRNA restored secretion (c). (d–f) Statistical evaluation of frequency of PNA (d) and serotonin (e) positive granules per SSC. The number of SSCs per area is shown in (f). Statistical significance was calculated by Wilcoxon test. See Table S1 for p values Image published in: Kurrle Y et al. (2020) Copyright © 2020. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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