Xenbase Image ID: 125785

Figure 1. Transient transfection to induce Vpr expression destabilizes both endogenous and GFP-CENP-A.A. Manual quantitation of GFP-CENP-A cells after transfection with control Vpr mutant W54R or wild-type Vpr. B–C. Example images of cells transfected with control Vpr-W54R-HA (B-B) or wild-type Vpr-HA (C-C) detected with anti-HA in red, DNA in blue, and GFP-CENP-A in green. GFP-CENP-A channel is also shown alone (B and C). D–E. Example fields of live GFP-CENP-A cells transfected with Vpr-W54R (D) or wild-type Vpr (E). F–G. Example fields of fixed HeLa cells after transfection with Vpr-W54R (F-F) or wild-type Vpr (G-G). Vpr is detected with anti-HA in green. Endogenous CENP-A is detected with anti-centromere autoantisera (ACA) in red. Image published in: Zeitlin SG et al. (2011) Zeitlin et al. Creative Commons Attribution license Larger Image Printer Friendly View

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