XB-IMG-117057
Xenbase Image ID: 117057
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Figure 8. Effect of extracellular trypsin on Na+ channel activity in outside-out excised patches. (a) In this original recording, amiloride (200 nM) added to the bath (extracellular side) solution, reduced the activity of a single Na+ channel, an effect that was quickly reversible. (b) With the same patch, trypsin (5 μg/ml) added to the external solution did not produce any obvious increase either in the single channel conductance or in the channel activity. The pipette was filled with a K+ gluconate solution and the bath solution contained 100 mM LiCl solution. The pipette potential was −100 mV. A downward deflection indicates a current flowing into the pipette and the closed-channel level is indicated by the dotted line. (c and d) The n.Po values in four patches in which recordings were obtained before, during, and after exposure to amiloride (c) and before and during exposure to trypsin (d) are shown. Image published in: Chraïbi A et al. (1998) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |