Xenbase Image ID: 159718

Fig. 3. Highly dynamic caspase activity during eye development. (A–F) Cryosections of the eyes in DEVD reporter X. laevis tadpoles at (A-C) stage 36 and (D–F) stage 45+. Nuclei are counterstained with DAPI. (A–C) A DEVD transgenic embryo at stage 36 showed consistent GFP signals in all retinal layers including the ganglion cell layer, the inner nuclear layer and the outer nuclear layer. At this stage, nuclei are still present in the differentiating lens fiber cells. (B) Higher magnification view of the GFP-positive photoreceptors in the box labeled ‘b’ in A. (C) Higher magnification view of the GFP-positive neuronal network between the different retinal layers in the box labeled ‘c’ in A. (D,E) GFP is expressed in the enucleated lens fiber cells of stage 45+ embryos (a higher magnification view of the box labeled ‘e’ is shown in E). A few GFP-positive cells were still observed in the fully developed retina (i.e. photoreceptor layer) (a higher magnification view of the box labeled ‘f’ is shown in F. Image published in: Tran HT et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View

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