Xenbase Image ID: 86786

Fig.2. The AP-1 binding site has a critical role in dominant-negative BMP receptor (DNBR)-induced FoxD5b expression. (A) Schematic representation of FoxD5b serially truncated constructs and a point mutation of AP-1 and Vent-1 putative binding site constructs (B). Embryos were co-injected with the -1336 construct (20pg) and DNBR (1ng) at the one-cell stage and incubated until stage 10. Luciferase activity was measured as described in Methods. (C) Various truncated constructs (20pg) were either injected alone or co-injected with DNBR (1ng) at the one-cell stage. Animal cap explants were dissected at stage 8, incubated until stage 10 and then luciferase activity was measured. (D) The -1336, AP-1 or Vent-1 site mutants were either injected alone or co-injected with DNBR (1ng) at the one-cell stage. Animal caps were dissected at stage 8, and their luciferase activity was measured at stage 10. (E) A chromatin immunoprecipitation (ChIP) assay was performed using Xenopus embryos at stage 10. The presence of the FoxD5b promoter was detected by PCR in DNA samples obtained from anti-c-Jun antibody precipitation (c-Jun), normal IgG antibody precipitation (IgG), and cross-linked chromatin supernatant before immunoprecipitation (Input). RLU, relative luciferase activity. *, p value < 0.05, **, p value < 0.01; ***, p value < 0.001. Image published in: Yoon J et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher. Larger Image Printer Friendly View

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