XB-IMG-124954
Xenbase Image ID: 124954
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Figure 8. Relationship between L520C:C445V:C449V fluorescence signal and ionic conductance.(A) Representative current and fluorescence traces recorded using the same protocol as in Fig. 2A. (B) The F-V relationship was obtained by plotting the amplitude of fluorescence at the end of the depolarization pulse against voltage (diamonds; V½ = −26.8±1.0 mV, k = 13.3±0.6 mV (n = 10)), and is compared to mean data for G-V (squares; V½ = −28.4±0.7 mV, k = 9.0±0.2 mV (n = 6)). (C) Comparison of time course of activation of E519C:S620T:C445V:C449V channels (circles, from Fig. 4C) with the time course of fluorescence deflection from L520C:C445V:C449V mutant (diamonds, n = 6). The fluorescence signal upon depolarization was fitted with a single exponential and plotted against voltage. Image published in: Es-Salah-Lamoureux Z et al. (2010) Es-Salah-Lamoureux et al. Creative Commons Attribution license Larger Image Printer Friendly View |