Xenbase Image ID: 125168

Figure 7. Loss of MyoD and Myf5 ablates somatic fast muscle and knockdown of GATA4 and GATA6 results in loss of GFP signal in the liver and intestine. (A) P1(−4885/−13)-GFP, P3(−3014/−1)-GFP and P3(−1292/−1)-GFP were separately injected or each coinjected with myod/myf5 double MOs into zebrafish embryos at the one-cell stage. Alternatively, myod/myf5 double MOs were injected to the transgenic Tg(Nogo-B:GFP) line at the one-cell stage. Zebrafish embryos at 48 hpf with GFP signals were selected for image analysis. Merged bright-field and fluorescence images are shown in panels (a’–h’), while fluorescence images are shown in panels (a–h). Scale bars indicate 100 μm. (B) Those embryos mentioned above at 3 dpf were subjected to whole-mount in situ hybridization using GFP as probe. All myod/myf5 double morphants did not show GFP signal. (C) GATA4/GATA6 double MOs were injected into zebrafish embryos of transgenic Tg(Nogo-B:GFP) line at one- to two-cell stage. The GATA4/GATA6 double-morphants and the parental transgenic line at 4 dpf were subjected to whole-mount in situ hybridization using GFP (panels a and b) and LFABP/iFABP (panels c and d) as probe. The liver and intestine were enlarged in panels (a’–d’). Image published in: Chen YC et al. (2010) © The Author(s) 2010. Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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