Xenbase Image ID: 117377

Figure 1. F-actin network movement in stationary and motile keratocytes. (A and B) Keratocytes were fixed and stained with phalloidin to visualize F-actin. (A) F-actin in stationary keratocytes was denser near the cell body than the periphery. (B) Motile keratocytes had a criss-cross pattern of F-actin staining in the lamellipodia and F-actin bundles at the cell rear. (C and E) AF546-phalloidin FSM images of F-actin networks in live stationary (C) and motile (E) keratocytes. (D and F) F-actin flow field relative to the substratum extracted by multiframe correlation tracking of speckle movement. Vectors indicate network displacements between consecutive frames. Color encodes flow speed. (D) The F-actin network in stationary keratocytes flowed centripetally inwards. (F) The F-actin network in motile keratocytes had a small retrograde flow in the lamellipodium and large inwards movement at the rear sides. (G) During symmetry breaking and motility initiation of keratocytes, the cell must transition from being stationary with radially symmetric centripetal actin flow to being polarized with decreased actin flow speed at the cell front. C and E are available as Videos 1 and 2 (available at http://www.jcb.org/cgi/content/full/jcb.200706012/DC1). Image published in: Yam PT et al. (2007) Copyright © 2007, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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