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Figure 2. Definition of GAT1-mediated currents and I–V relations. (A) Chart record of membrane current at 0 mV: extracellular and cytoplasmic solutions were as indicated in millimolar. Outward GAT1 current was activated by applying 20 mM cytoplasmic GABA in the presence of 120 mM cytoplasmic NaCl. After washing off all cytoplasmic substrates with 120 mM NMG-MES, extracellular solution was changed to 120 mM NaCl via pipette perfusion. Inward GAT1 current was then turned on and off by adding and removing 0.2 mM GABAo from the pipette solution. (B) I–V protocol and the corresponding membrane current responses at the time points indicated in A. (C) I–V relations of GABA-defined outward and inward GAT1 current. The median current amplitudes during the last 3 ms of each voltage step in B are plotted against membrane potential.

Image published in: Lu CC and Hilgemann DW (1999)

© 1999 The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license

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