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Figure 4. Appearance of 5-HT3AB receptor characteristics differ following transfection with 5-HT3B or 5-HT3Br1 subunits. Cells were transfected with 5-HT3A subunit cDNA (20 ng per well) and various amounts of 5-HT3B or 5-HT3Br1 subunit DNA, and incubated for 2 (A) or 3 (B) days. Higher EC50 and lower nH values (i.e., 5-HT3AB receptor characteristics) were observed in cells incubated for 2 days with 2 and 20 ng of 5-HT3B subunit cDNA, but those transfected with 0.2 ng of cDNA had responses with characteristics consistent with homomeric 5-HT3A receptors. Cells transfected with 200 ng of 5-HT3BR1 subunit cDNA had 5-HT3AB receptor characteristics after 3 days of incubation. Responses with characteristics consistent with homomeric 5-HT3A receptors were observed for cells transfected with 2 or 20 ng of cDNA, and for cells incubated for 2 days (data not shown). Data = mean ± SEM, n = 3–6;*significantly different from 5-HT3A receptor responses. Image published in: Corradi J et al. (2015) Image downloaded from an Open Access article in PubMed Central. Copyright © 2015 American Chemical Society Larger Image Printer Friendly View

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