XB-IMG-122820
Xenbase Image ID: 122820
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Fig. 2. ATP-inhibition of L164P channels is less that that of wild-type channels. a, c Currents recorded in inside-out patches excised from Xenopus oocytes expressing hetKir6.2-L164P/SUR1 (hetL164P) or homKir6.2-L164P/SUR1 (homL164P) channels, as indicated, in response to 3 s voltage ramps from −110 to +100 mV. ATP (10 mmol/l) was applied as indicated by the horizontal bars in the absence (a) or presence (c) of 2 mmol/l Mg2+. b, d, mean relationship between [ATP] and KATP conductance (G), expressed relative to the conductance in the absence of the nucleotide (Gc), for wild-type (white circles, n = 9), hetL164P (white/black circles, n = 6) or homL164P (black circles, n = 4) channels. Experiments were carried out in the absence (b) or presence (d) of 2 mmol/l Mg2+. The continuous lines through the black circles were drawn by eye. The smooth curves are the best fit to the Hill equation with IC50 of 11 μmol/l (wild-type) and 100 μmol/l (hetL164P) (b, 0 mmol/l Mg2+) or IC50 of 16 μmol/l (wild-type) and 122 μmol/l (hetL164P) (d, 2 mmol/l Mg2+) Image published in: Tammaro P et al. (2008) Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2008 Larger Image Printer Friendly View |