XB-IMG-122908
Xenbase Image ID: 122908
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Figure 7. Nonacidic residues also contribute to Cu2+ sensitivity. (A) IC50s of mutant channels are plotted at each position tested for Cu2+ (open symbols) or Cd2+ (filled symbols). Error bars were within the symbol size and therefore excluded. Symbols indicate different substitutions (WT: , ; Ala: â, â¢; Lys: â¡; Gln: â; Asn: â; Cys: â¿,â¾; His: âµ, â´). Dashed line indicates the IC50 of the WT for Cu2+. Vertical solid lines indicate the differences in IC50 between mutants at putative coordination sites. Doseâresponse relations (GK-[Cu2+]) for putative coordination sites 151 and 207 in 0 Ca2+ are plotted and fit by Hill equations in (B) Q151A (â, IC50 = 23.8 μM, nH =1.0), Q151C (<, IC50 = 0.96 μM, nH = 0.97) at +240 mV and (C) R207Q (â, IC50 = 85.8 μM, nH = 0.77) and R207C (<, IC50 = 0.52 μM, nH = 0.77) at +180 mV. Image published in: Ma Z et al. (2008) © 2008 Ma et al. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |