XB-IMG-126793
Xenbase Image ID: 126793
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Figure 4. Development of a thallium flux assay for Kir2.3. (A) Thallium flux-dependent FluoZin-2 fluorescence recorded from monoclonal Kir2.3 T-REx-HEK-293 cells cultured overnight in absence (−Tet) or presence (+Tet) of Tetracycline. The fluorescence emission was recorded before and after the addition of extracellular thallium (shaded box). (B) Representative traces for changes of Tl+-induced FluoZin-2 fluorescence following 20 min pre-treatment of cells with the indicated concentrations of VU573. (C) CRC for VU573-dependent inhibition of Kir2.3 activity. Values are mean ± SEM (n = 3). A fit of the CRC with a single-site four-parameter logistic function yielded IC50 of 4.7. Image published in: Raphemot R et al. (2011) Copyright © 2011 Raphemot, Lonergan, Nguyen, Utley, Lewis, Kadakia, Weaver, Gogliotti, Hopkins, Lindsley and Denton. Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View |