Xenbase Image ID: 127644

Figure 1. Transient peptide-activated currents depend on Cl−. (A) Representative current trace showing activation of HyNaC2/3/5 in standard bath. HyNaC was activated with 1 µM Hydra-RFamide I (black bars). (B) Repeated activation of HyNaC at different holding potentials (Vhold) reveals the different Erevs of the transient and the sustained current component. A representative current trace in standard bath solution is shown. Note that the transient current changes to outward at more negative potentials than the sustained current. The inset highlights the three current phases (initial inward, transient outward, and sustained inward) at a holding potential of −20 mV. (C) I/V plot revealing the different Erevs of the transient (open squares) and sustained current components (closed squares) in standard bath (n = 6). Note the strong inward rectification of both components. The inset shows the I/V relations close to the Erevs on an expanded scale. (D) Representative current trace in bath solution lacking Cl− (NaCl replaced by NaGluconate). Note the absence of transient outward currents. Image published in: Dürrnagel S et al. (2012) © 2012 Dürrnagel et al. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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