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Figure 9. . PIP2 and PKA are required for restoration of SK channel activity after purinergic receptors mediated inhibition by extracellular Mg-ATP. Cell-attached apical membrane patches were obtained, extracellular Mg-ATP (0.2 mM) was added to the bath, and then patches were excised in the inside-out configuration. Representative SK channel traces are shown demonstrating inhibition of SK activity by extracellular Mg-ATP in the cell-attached configuration. In the inside-out configuration, no channel activity was observed in the absence of Mg-ATP or PIP2 (A). However, with addition of 0.5 mM Mg-ATP followed by 50 U of the catalytic subunit of PKA (B) SK channels were nearly completely reactivated. C = channel-closed state. −Vpipette = pipette-holding potential.

Image published in: Lu M et al. (2002)

Copyright © 2002, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license

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