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XB-IMG-178517

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Figure S6: (A) Rnf20 expression in the mouse LRO. The LRO structure was identified by presence of monociliated cells labeled with anti-Arl13b, nuclei were visualized by Hoechst staining. Anterior (A)-Posterior (P) and Left (L)-Right (R) axes are indicated. The image is the maximum intensity projection of a Z stack encompassing the entire LRO. (B) RNF20 expression in mouse multiciliated tissues visualized by immunostaining with anti-RNF20 antibody (Green). Multiciliated cells were identified by ciliary labeling with anti-acetylated tubulin (Red), and nuclei by Hoechst staining. (C) Western blot of 20µg/lane adult mouse tissue probed with anti-Rnf20 antibody showing expected 110kD band. Loading was normalized to total protein determined by BCA assay and confirmed by SimplyBlue SafeStain (Coomassie G-250).

Image published in: Robson A et al. (2019)

Copyright © 2019. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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