Xenbase Image ID: 122743

Figure 5. Flux studies. X. laevis oocytes were injected with saNKCC2A, saNKCC2F, saNKCCC2AF, saNKCC2A + saNKCC2AF, or saNKCC2F + saNKCC2AF (∼2 ng for A or F, and ∼20 ng for AF). Groups of eggs were also injected with H2O alone to determine background cotransporter activity. (A and B) 86Rb influx measured in 13–22 oocytes expressed as means ± SEM. Bumetanide and ouabain were added to the influx and wash solution at a concentration of 250 μM and 10 μM. (C) 22Na influx measured in 9–10 oocytes expressed as means ± SEM. Hydrochlorothiazide, amiloride, and ouabain were added to the influx and wash solution at concentrations of 250, 125, and 125 μM. Here, * indicates that within any given panel, the values are significantly different from those of A or F (P < 0.01). Image published in: Brunet GM et al. (2005) Copyright © 2005, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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