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XB-IMG-121257

Xenbase Image ID: 121257


Figure 3. . Substrate dose responses for C-C mutant and WT before and after modification with MTSEA. (A) Pi activation for WT (open diamonds) and C-C mutant (filled squares) before modification with MTSEA at Vh = −50 mV, measured in ND100 with Pi as variable substrate. For the C-C mutant, data are also shown after incubation with MTSEA (open squares), by measuring the change in holding current induced by Pi relative to the holding current in 0 mM Pi. Each data point represents mean ± SEM (n = 4). The modified Hill equation was fit to the data to give: KmPi = 0.06 ± 0.010 (C-C, −MTSEA); KmPi = 0.06 ± 0.01 (C-C, +MTSEA) and KmPi = 0.060 ± 0.01 (WT), nH was constrained to 1.0 for fit. (B) Na+ activation for WT (open diamonds) and C-C mutant (filled squares) before modification with MTSEA at Vh = −50 mV measured at 1 mM Pi with Na+ as variable substrate. For the C-C mutant, data are also shown after incubation with MTSEA (open squares) by measuring the change in holding current induced by Pi relative to the current in 0 mM Pi. Data were pooled as in A (n = 4); fit of Hill equation gave: KmNa = 52 ± 8 (C-C, −MTSEA), KmNa = 55 ± 6 (C-C, +MTSEA), KmNa = 61 ± 10 (WT), nH = 2.3, 2.7, and 2.1, respectively. Points without error bars have SEMs smaller than the symbol size.

Image published in: Köhler K et al. (2002)

Copyright © 2002, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license

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