XB-IMG-124821
Xenbase Image ID: 124821
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Figure 3. The ATR–9-1-1 pathway is not required for primer synthesis. (A–D) Sperm chromatin (2,000 nuclei/µl) was replicated in mock- or ATRIP-depleted LSE with or without 15 µM aphidicolin (Aph). (A) Samples were immunoblotted to verify depletion. (B–D) Aliquots were taken at the indicated times and analyzed for replication (B), Chk1 phosphorylation (C), and primer synthesis (D) as in Fig 1. (E–G) Sperm chromatin (2,500 nuclei/µl) was replicated in mock- or Rad1-depleted LSE containing recombinant 9-1-1 complex and 15 µM aphidicolin as indicated. (E and G) Aliquots were analyzed for Chk1 phosphorylation (E) and primer synthesis (G) as in Fig. 1 A. (F) In parallel, chromatin was isolated from a third aliquot, and bound proteins were immunoblotted with the indicated antibodies. Image published in: Van C et al. (2010) © 2010 Van et al. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |