XB-IMG-122682
Xenbase Image ID: 122682
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Figure 4. Exceptional slowed gating kinetics in only the positive-charged 334R and 334K channels. (A) Parental control ΔC26 channels without SUR show fast burst exit gating, observed as very short bursts of one to several openings and a PO ∼0.08. (B) For arginine at position 334, 6 of 11 patches exhibited dramatically slowed gating. (C) For the lysine mutant, 6 of 10 patches showed the fast gating, and 4 of 10 patches the slowed gating. On average, the exceptional slowed gating for either positive-charged substitution at 334 was characterized by approximately fivefold increase in PO, from ∼0.1 to ∼0.5. During these initial experiments, interconversions between the fast and slow gating conformations were not observed and could not be induced by a variety of bath solutions. Image published in: Li L et al. (2005) Copyright © 2005, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |