Xenbase Image ID: 125320

Figure 3. Proprotein processing in Ac45-transgenic melanotrope cells is less effectively inhibited by bafilomycin A1. (A and B) Neurointermediate lobes from wild-type (wt) and Ac45-transgenic (tg) animals were pulse labeled for 30 min and subsequently chased for 180 min in medium containing 0, 0.25, or 0.5 μM bafilomycine A1. Newly synthesized proteins were extracted from the lobes, directly analyzed on 15% SDS-PAGE to resolve the 37-kDa POMC and 18-kDa POMC products (A) and on 10% SDS-PAGE to resolve the various PC2 forms (B). Signals were visualized by autoradiography. Image published in: Jansen EJ et al. (2010) © 2010 by The American Society for Cell Biology. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page