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Figure 6. Relationship of relative permeability and alkali ionic radii for ChR2 serine mutations.Relative permeability ratios were calculated using reversal potentials for each mutant. (A) Plotting the square root of relative permeability ratios vs. ionic radius yielded a linear fit for each data set. Solid line/diamond, WT; dashed line/square, V269S; broken line/X S136A. For all mutants, the larger alkali metal ions (K+, Rb+, and Cs+) were less permeable than the smaller alkali metal ions (Li+, Na+). These results were consistent with excluded volume theory [36]. (B) Minimum pore fitting parameters and pore size calcualtions for single serine ChR2 mutants and S136A. The coefficients a and b correspond to the y-intercept and slope of the fitted data, respectively.

Image published in: Richards R and Dempski RE (2012)

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