Xenbase Image ID: 123348

Figure 3. Human SLC2A9a-Mediated Urate Efflux from Xenopus Oocytes(A) Comparison of urate and L-glucose efflux from SLC2A9a-expressing oocytes. Oocytes injected with SLC2A9a cRNA 4 d prior (triangles) or water-injected eggs (circles or squares) were injected with 14C-labelled urate (circles, triangles, or diamonds) or 14C-labelled L-glucose (filled squares) to give an estimated initial intracellular concentration of 200 μM. 20 oocytes per condition were incubated at 22 °C in efflux medium, which was sampled every 2 min. Efflux media contained 5 mM D-glucose (open circles), 5 mM L-glucose (filled squares, circles or triangles), or 2 mM urate (filled diamonds). Data points represent the log percentage of urate or L-glucose remaining in the oocytes for each time point. Lines were fitted by linear regression.(B) Acceleration of SLC2A9a mediated urate efflux by extracellular D-glucose or D-fructose. Oocytes injected with SLC2A9a cRNA 4 d prior (triangles) or water-injected eggs were injected with 14C-labelled urate to give an estimated intracellular concentration of 200 μM. 20 oocytes per condition were incubated at 22 °C in efflux medium, which was sampled every 2 min. Efflux media contained 5 mM D-glucose (filled circles), 5 mM L-glucose (filled triangles) or 5 mM D-fructose (open circles). Data points represent the log percentage of urate remaining in the oocytes for each time point corrected for the efflux of urate from the water-injected eggs under the same conditions. Lines were fitted by linear regression. Image published in: Caulfield MJ et al. (2008) Copyright: © 2008 Caulfield et al. Creative Commons Attribution license Larger Image Printer Friendly View

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