Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-IMG-120406

Xenbase Image ID: 120406


Figure 3. . Kinetics of depolarization-induced IRK1 block by bis-QAC10. (A) Current transient elicited by stepping membrane voltage from −100 to 70 mV in the absence (control) or presence of 0.1 μM bis-QAC10; the first 3 ms of the transient in the presence of bis-QAC10 was omitted (see results). The curve superimposed on the transient (and back extrapolated to the start of depolarization) is a single-exponential fit. (B) The reciprocal of the time constant (mean ± SEM, n = 6) for channel block (1/τon), obtained as shown in A, is plotted against bis-QAC10 concentration (0.1, 1, and 10 μM) for four voltages. The lines through the data are linear fits whose slope gives the apparent second-order rate constant (appkon) for blocker binding. (C) The natural logarithm of appkon from B is plotted against membrane voltage. The line through the data is a fit of the Boltzmann function, yielding kon(0 mV) = 1.63 ± 0.37 × 107 M−1s−1 (mean ± SEM, n = 6) and zon = 0.73 ± 0.07.

Image published in: Shin HG and Lu Z (2005)

Copyright © 2005, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license

Larger Image
Printer Friendly View

Return to previous page